Expression of cDNA for recombinant human granulocyte colony-stimulating factor inEscherichia coliand characterization of the protein
โ Scribed by Shu Zhang; Qinong Ye
- Book ID
- 105668848
- Publisher
- Chinese Anti-Cancer Association
- Year
- 1998
- Tongue
- English
- Weight
- 419 KB
- Volume
- 10
- Category
- Article
- ISSN
- 1000-9604
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A new mutant protein of recombinant human granulocyte colony-stimulating factor (rhG-CSF) was produced for the studies on receptors for human G-CSF. The mutant protein [(Tyr1, Tyr3]rhG-CSF), the biological activity of which was almost equal to that of rhG-CSF, was prepared by the replacement of thre
A human granulocyte colony-stimulating factor (hG-CSF) gene was syntheeized and inserted into a trp expression vector for overexpression in E. cm?. A strong expression vector was constructed, and a simple purification procedure including in vitro refolding was estabiliehed. The final productivity of