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Expression of Recombinant Human Epidermal Growth Factor inEscherichia coliand Characterization of its Biological Activity

โœ Scribed by Ahmad Faizal Abdull Razis; Elysha Nur Ismail; Zarida Hambali; Muhammad Nazrul Hakim Abdullah; Abdul Manaf Ali; Mohd Azmi Mohd Lila


Book ID
107564635
Publisher
Springer-Verlag
Year
2007
Tongue
English
Weight
196 KB
Volume
144
Category
Article
ISSN
0273-2289

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Human recombinant EGF, secreted into the extracellular medium by E. c&i cells, was purified by a combination of solid phase extraction and HPLC. Using these techniques, the peptide was purified 122-fold, with a recovery of greater than 75%. The purified hEGF manifested no contaminating protein bands