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Expression and purification of Clostridium perfringens beta-toxin glutathione S-transferase fusion protein

✍ Scribed by Valgerdur Steinpórsdótti; Vala Fridriksdóttir; Eggert Gunnarsson; ólafur S. Andrésson


Book ID
109320691
Publisher
John Wiley and Sons
Year
1995
Tongue
English
Weight
628 KB
Volume
130
Category
Article
ISSN
0378-1097

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Prokaryotic expression of polypeptides as fusion proteins with glutathione-S-transferase has recently been reported as a one-step means of purifying recombinant protein. The usefulness of the glutathione-S-transferase/glutathione- agarose system, however, is significantly limited by the frequent syn