Prokaryotic expression of polypeptides as fusion proteins with glutathione-S-transferase has recently been reported as a one-step means of purifying recombinant protein. The usefulness of the glutathione-S-transferase/glutathione- agarose system, however, is significantly limited by the frequent syn
Solubilization and Purification of Enzymatically Active Glutathione S-Transferase (pGEX) Fusion Proteins
โ Scribed by J.V. Frangioni; B.G. Neel
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 876 KB
- Volume
- 210
- Category
- Article
- ISSN
- 0003-2697
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