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Secretion and affinity purification of glutathioneS-transferase fusion proteins from yeast

โœ Scribed by L. A. Castelli; A. J. Petris; S. M. Carroll; I. G. Macreadie


Publisher
Springer-Verlag
Year
1995
Tongue
English
Weight
542 KB
Volume
9
Category
Article
ISSN
0951-208X

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๐Ÿ“œ SIMILAR VOLUMES


Affinity purification of insoluble recom
โœ John Hartman; Paru Daram; Raymond A. Frizzell; Thomas Rado; Dale J. Benos; Eric ๐Ÿ“‚ Article ๐Ÿ“… 1992 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 647 KB

Prokaryotic expression of polypeptides as fusion proteins with glutathione-S-transferase has recently been reported as a one-step means of purifying recombinant protein. The usefulness of the glutathione-S-transferase/glutathione- agarose system, however, is significantly limited by the frequent syn