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Expression and Characterization of Phosphorylated Recombinant Human β-Casein inEscherichia coli

✍ Scribed by Jennifer M. Thurmond; Robert G. Hards; C.Thomas Seipelt; Amanda E. Leonard; Lennart Hansson; Mats Strömqvist; Mona Byström; Kerstin Enquist; Bixiong C. Xu; John J. Kopchick; Pradip Mukerji


Book ID
115645173
Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
256 KB
Volume
10
Category
Article
ISSN
1046-5928

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Human 17beta-hydroxysteroid dehydrogenase type 1 (17beta-HSD1) catalyzes the reaction of estrone with NADPH to form estradiol and NADP(+), thereby regulating the biological activity of sex steroid hormones in a variety of tissues. Here, we present an efficient method for expressing and purifying hum