Exploration of sulfur metabolism in the yeastKluyveromyces lactis
✍ Scribed by Agnès Hébert; Marie-Pierre Forquin-Gomez; Aurélie Roux; Julie Aubert; Christophe Junot; Valentin Loux; Jean-François Heilier; Pascal Bonnarme; Jean-Marie Beckerich; Sophie Landaud
- Publisher
- Springer
- Year
- 2011
- Tongue
- English
- Weight
- 398 KB
- Volume
- 91
- Category
- Article
- ISSN
- 1432-0614
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Major proteolytic activities were characterized in the yeast K. lactis NRRL 1118, grown in chemostat cultures. This yeast expressed proteolytic activities similar to those found in S. cerevisiae. This fact was particularly evident in the case of proteases such as PrA, PrB and CpY with regard to subs
The killer character of Kluyveromyces lactis is associated with a cytoplasmic genetic element which confers on the host cells: a) the capacity to kill sensitive cells of various yeast by producing a diffusible toxin, and b) the immunity to that toxin. The killing activity is associated with the pres
We have studied the structure of the two linear DNA plasmids, kl and k2, present in killer strains of Kluyveromyces lactis. Two killer strains of different origins, CBS 2359 and IFO 1267 were examined. For both strains, identical restriction maps of kl and k2 DNA were obtained. Several restriction s
The killer character of Kluyverornyces lactis is cytoplasmically inherited. Two DNA plasmids kl and k2 (8.8 and 13.4 kilobase pairs, respectively) are associated with the killer system. Either cytoplasmic or nuclear mutations can lead to a loss of the killer activity. Two cytoplasmic non-killer muta