Experimental determination of the free energy of unfolding of proteins
โ Scribed by Saad Tayyab; Mashiat Ullah Siddiqui; Nisar Ahmad
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 283 KB
- Volume
- 23
- Category
- Article
- ISSN
- 0307-4412
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## Abstract In this article we use literature data on the titration of denatured ribonuclease to test the accuracy of protonโbinding distributions obtained using our recent approach employing moments. We find that using only the local slope of the titration curve at a small number of points (five,
Protein unfolding occurs when the balance of forces between the protein's interaction with itself and the protein's interaction with its environment is disrupted. The disruption of this balance of forces may be as simple as a perturbance of the normal water structure around the protein. A decrease i