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Exclusion of spheres by agarose gels during agarose gel electrophoresis: Dependence on the sphere's radius and the gel's concentration

✍ Scribed by Philip Serwer; Shirley J. Hayes

Publisher: Elsevier Science
Year: 1986
Tongue: English
Weight: 641 KB
Volume: 158
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(86)90591-9

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✦ Synopsis

Agarose gel electrophoresis of spheres (radius = R) has been used to determine the effective radius (PE) of the pores of an agarose gel (percentage of agarose in a gel = A). The value of PE at a given A was taken to be the R of the largest sphere that enters the gel. When log PE is plotted as a function of log A, the results can be represented by: PE = 118A-"."' for 0.2 4 A < 4.0 (PE in nm). However, the data suggest significant nonlinearity in this plot, the magnitude ofthe exponent of the PE vs A relationship increasing by about 20% as A increases from 0.2 to 4.0. From these data, PE's as big as 1500 nm and as small as 36 nm can be achieved with agarose gels formed with unmodified, unadulterated agarose and usable for electrophoresis.

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