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Electrophoresis of 35S-labeled proteoglycans on polyacrylamide-agarose composite gels and their visualization by fluorography

✍ Scribed by Stephen L. Carney; Michael T. Bayliss; Julia M. Collier; Helen Muir


Publisher
Elsevier Science
Year
1986
Tongue
English
Weight
626 KB
Volume
156
Category
Article
ISSN
0003-2697

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✦ Synopsis


We have developed techniques for the electrophorcsis of "S-labeled proteoglycans on polyacrylamide-agarose gel slabs and subsequent fixation. impregnation, and fluorography of such electrophoretograms.

The procedure permits the examination of newly synthesized proteoglycan subspecies using a rapid technique, previously unavailable for these labeled molecules. IC 1986