C nuclear magnetic resonance spectroscopy was used to determine the absolute amounts of de novo synthesized amino acids in both the perchloric acid extracts and the hydrolyzed protein fractions of F98 glioma cells incubated for 2 h with 5 mmol/l [U-13 C]glucose. 13 C NMR spectra of the hydrolyzed pr
Exchange Rate Constants of Invisible Protons in Proteins Determined by NMR Spectroscopy
β Scribed by Takuya Segawa; Fatiha Kateb; Luminita Duma; Geoffrey Bodenhausen; Philippe Pelupessy
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- English
- Weight
- 78 KB
- Volume
- 10
- Category
- Article
- ISSN
- 1439-4227
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## Abstract The Ο angle in a cyclic peptide is determined by the combined use of homonuclear and heteronuclear coupling constants. Two of the four coupling constants that define the Ο angle in a peptide are determined exactly, two qualitatively. Via Karplusβtype equations, they are transformed into