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Two-compartment model for determination of glycolytic rates of solid tumors by in vivo13C NMR spectroscopy

✍ Scribed by Dmitri Artemov; Zaver M. Bhujwalla; Ulrich Pilatus; Jerry D. Glickson

Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
125 KB
Volume
11
Category
Article
ISSN
0952-3480

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✦ Synopsis

Carbon-13 NMR spectroscopy of 13 C enriched substrates is useful for non-invasively determining metabolic fluxes of cells and tissues. Our study demonstrates that for RIF-1 tumor cells, examined under monolayer culture with continuous perfusion and also grown as solid subcutaneously (sc) implanted tumors in vivo, the levels of intracellular glucose and intermediates of the glycolytic pathway are below the level of detection by NMR spectroscopy. For these tumors, glucose transport into the cell is the most probable rate limiting step of the glycolytic pathway. Under these limiting conditions a simple two-compartment model of glycolysis applies. This model yields two parameters: the average rate of glycolysis and the rate of lactate clearance through the vasculature. For the RIF-1 tumor these parameters were 0.022 AE 0.01 and 0.034 AE 0.006 min Γ€1 , respectively.

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