Examination of isoelectric focusing and electrophoretic methods for resolving acidic proteins
β Scribed by Eric D. Green; David A. Knecht; Randall L. Dimond
- Book ID
- 102834543
- Publisher
- John Wiley and Sons
- Year
- 1986
- Tongue
- English
- Weight
- 818 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0173-0835
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
A new set-up was constructed for capillary isoelectric focusing (CIEF) involving a sampling capillary as a bypass fixed to the separation capillary. Sample solutions were subjected to a previously established pH gradient from the sample capillary. Besides performing conventional CIEF, the separation
## Abstract We present here a protocol for unidirectional wet electrotransfer of isoelectric focusing (IEF) gels to polyvinylidene difluoride (PVDF) that can be carried out in < 2 h, including preequilibration steps. In developing this protocol, we systematically evaluated sodium dodecyl sulfate an