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Protocol of capillary isoelectric focusing to separate extremely acidic and basic proteins

✍ Scribed by Chun Yang; Weibing Zhang; Jie Zhang; Jicheng Duan; Yukui Zhang

Publisher
John Wiley and Sons
Year
2005
Tongue
English
Weight
330 KB
Volume
28
Category
Article
ISSN
1615-9306

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✦ Synopsis

A new set-up was constructed for capillary isoelectric focusing (CIEF) involving a sampling capillary as a bypass fixed to the separation capillary. Sample solutions were subjected to a previously established pH gradient from the sample capillary. Besides performing conventional CIEF, the separation of ampholytic compounds with isoelectric points (p/s) beyond the pH gradient was carried out on this system. This method was termed as pH gradient driven electrophoresis (PGDE) and the basic mathematical expressions were derived to express the dynamic fundamentals. Proteins such as lysozyme, cytochrome C, and pepsin with p/s higher than 10 or below 3 were separated in a pH gradient provided by Pharmalyte (pH 3-10). Finally, this protocol convincingly exhibited its potential in the separation of a solution of chicken egg white.

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