✦ LIBER ✦

Method for rapid electrophoretic transfer of isoelectric focusing gels to polyvinylidene difluoride

✍ Scribed by Dr. Sharon Lobert; John J. Correia

Publisher: John Wiley and Sons
Year: 1994
Tongue: English
Weight: 199 KB
Volume: 15
Category: Article
ISSN: 0173-0835
DOI: 10.1002/elps.11501501135

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✦ Synopsis

Abstract

We present here a protocol for unidirectional wet electrotransfer of isoelectric focusing (IEF) gels to polyvinylidene difluoride (PVDF) that can be carried out in < 2 h, including preequilibration steps. In developing this protocol, we systematically evaluated sodium dodecyl sulfate and methanol concentrations in the preequilibration washes and length of electrotransfer. We demonstrated that the method is useful for tubulin, a protein that resists usual semi‐dry transfer procedures due to its limited solubility under IEF conditions. We also successfully used our method for transferring the microtubule‐associated protein, tau, as well as carbamylyte standards, creatine phosphokinase and carbonic anhydrase.

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