✦ LIBER ✦

Evidence that the phr + gene enhances the ultraviolet resistance of Escherichia coli recA strains in the dark

✍ Scribed by Yamamoto, Kazuo ;Fujiwara, Yoshisada ;Shinagawa, Hideo

Publisher: Springer
Year: 1983
Tongue: English
Weight: 226 KB
Volume: 192
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00327679

No coin nor oath required. For personal study only.

✦ Synopsis

An Escherichia coli recA phr+ purA strain was more resistant to ultraviolet radiation than its isogenic derivative recA phr+ purA+ in the absence of photoreactivating light, whereas their nearly isogenic derivative recA phr showed most UV-induced lethality. The amounts of photoreactivating enzyme (PRE) per cell in the recA phr+ purA was higher than in the recA phr+ purA+. The recA phr is defective for photoreactivation. Thus, in the recA strain, UV resistance in the dark increased in proportion to the amounts of PRE per cell, suggesting that PRE participates in the process of dark repair of UV-damaged DNA.

📜 SIMILAR VOLUMES

The Escherichia coli recA gene increases

The Escherichia coli recA gene increases resistance of the yeast Saccharomyces cerevisiae to ionizing and ultraviolet radiation

✍ Brozmanová, Jela ;Černáková, Ľubica ;Vlčková, Viera ;Duraj, Jozef ;Fridrichová, 📂 Article 📅 1991 🏛 Springer 🌐 English ⚖ 947 KB

The Escherichia coli recA protein coding region was ligated into an extrachromosomally replicating yeast expression vector downstream of the yeast alcohol dehydrogenase promoter region to produce plasmid pADHrecA. Transformation of the wild-type yeast strains YNN-27 and 7799-4B, as well as the recom

Altered regulation of the recA gene in E

Altered regulation of the recA gene in Escherichia coli strains carrying a recA-linked suppressor of lexA

✍ Thomas, A. ;Lloyd, R. G. 📂 Article 📅 1980 🏛 Springer 🌐 English ⚖ 297 KB

A mutant strain of E. coli displaying altered regulation of the recA gene was isolated as a revertant of a lexA3 recA200 double mutant which showed improved DNA repair and recombination functions. The mutation (alc-24) was located between srl and recA200 and caused synthesis of high levels of recA p

Amelioration of the ultraviolet sensitiv

Amelioration of the ultraviolet sensitivity of an Escherichia coli recA mutant in the dark by photoreactivating enzyme

✍ Yamamoto, Kazuo ;Satake, Mitsunobu ;Shinagawa, Hideo ;Fujiwara, Yoshisada 📂 Article 📅 1983 🏛 Springer 🌐 English ⚖ 568 KB

When a recA strain of Escherichia coli is transformed with a multicopy plasmid, pKY1, carrying the phr gene of E. coli, its extreme ultraviolet sensitivity is decreased. Derivatives of pKY1 were prepared in which the phr gene was inactivated by inserting transposon Tn1000. None of the 20 phr- deriva

Evidence that a system similar to the re

Evidence that a system similar to the recA system of Escherichia coli exists in Vibrio cholerae

✍ Ghosh, R. K. ;Siddiqui, K. A. I. ;Mukhopadhyay, G. ;Ghosh, Amit 📂 Article 📅 1985 🏛 Springer 🌐 English ⚖ 297 KB

Two lines of evidence suggest that a gene analogous to the recA gene of Escherichia coli exists in Vibrio cholerae and that its product serves a proteolytic function in the SOS response. Firstly, Southern blot hybridization using the recA gene of E. coli as a probe revealed a genomic sequence in V.

Molecular organisation of the genes invo

Molecular organisation of the genes involved in the production of F72 fimbriae, causing mannose resistant haemagglutination, of a uropathogenic Escherichia coli 06:K2:H1:F7 strain

✍ van Die, Irma ;van Megen, Ingrid ;Hoekstra, Wiel ;Bergmans, Hans 📂 Article 📅 1984 🏛 Springer 🌐 English ⚖ 901 KB

The genes responsible for the formation of the F7 (2) fimbriae of the uropathogenic E. coli strain AD110 (O6:K2:H1: F7 ) have been cloned on the recombinant plasmid pPIL110 -35 (Van Die et al. 1983). The F7 (2) fimbriae, like the F7 (1) fimbriae of AD110 , are responsible for mannose resistant haema