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Evidence for Semiconservative Replication of Circular Polyoma Dna

โœ Scribed by Bernhard Hirt


Book ID
123647420
Publisher
National Academy of Sciences
Year
1966
Tongue
English
Weight
967 KB
Volume
55
Category
Article
ISSN
0027-8424

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Polyoma DNA was cleaved with restriction endonuclease HpaII, the fragments were separated by gel electrophoresis and transferred in good yield to separate nitrocellulose filters by a modification of the procedure of E. M. Southern (1975,J. Mol. Biol. 98,503-517.) The filters were then used in hybrid