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The secondary structure of replicating polyoma virus DNA

โœ Scribed by Danielle Bourgaux-Ramoisy

Book ID
115744656
Publisher
Elsevier Science
Year
1971
Weight
207 KB
Volume
254
Category
Article
ISSN
0005-2787

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Polyoma DNA was cleaved with restriction endonuclease HpaII, the fragments were separated by gel electrophoresis and transferred in good yield to separate nitrocellulose filters by a modification of the procedure of E. M. Southern (1975,J. Mol. Biol. 98,503-517.) The filters were then used in hybrid