✦ LIBER ✦

Evidence for a Mendelian factor controlling the cytokinin requirement of cultured tobacco cells

✍ Scribed by Meins, Frederick ;Foster, Rachel ;Lutz, Joseph D.

Publisher: John Wiley and Sons
Year: 1983
Tongue: English
Weight: 697 KB
Volume: 4
Category: Article
ISSN: 0192-253X
DOI: 10.1002/dvg.1020040207

No coin nor oath required. For personal study only.

✦ Synopsis

Cultured leaf tissues of Nicotiana tabacum L. cv. "Havana 425" normally require an exogenous source of cytokinin for rapid growth; stem-cortex tissues do not-ie, they exhibit the cytokinin-habituated phenotype. We found that plants regenerated from cloned cortex and leaf tissues from one particular plant differed in leaf-tissue phenotype: Leaf tissues derived from leaf cells exhibited the normal, nonhabituated phenotype, whereas leaf tissues derived from cortex cells were cytokinin-habituated. This difference in leaf phenotype was not found using leaf and cortex cells from six other donor plants. The inheritance of the habituated leaf trait was studied in tissues from cortex-derived plants and hybrids between these plants and normal plants. F, hybrids were intermediate between the parental types in degree of habituation. No differences were found between reciprocal hybrids. These results suggest that the habituated leaf trait is an incompletely dominant, nuclear trait. Both parental and intermediate phenotypes were recovered in the F2 progeny. The frequency of habituated leaf progeny in the F2 and backcross populations provide evidence that the trait is regulated at a single genetic locus.

📜 SIMILAR VOLUMES

A serum factor requirement for the passa

A serum factor requirement for the passage of cultured Vero cells through G2

✍ Dean L. Engelhard; Mao Jen-Hao 📂 Article 📅 1977 🏛 John Wiley and Sons 🌐 English ⚖ 978 KB

## Abstract When Vero cells, a line derived from an African Green Monkey kidney, are grown under conditions where the saturation density is limited by serum, they deplete the growth medium of a factor necessary for cell division. The factor is a component of serum. When Vero cells are plated at low

Studies on the control of plasminogen ac

Studies on the control of plasminogen activator production by cultured human embryonic lung cells: Requirements for inhibition by corticosteroids

✍ Daniel B. Rifkin; Ruth M. Crowe 📂 Article 📅 1980 🏛 John Wiley and Sons 🌐 English ⚖ 503 KB

## Abstract The ability of actinomycin D to interfere with the dexametha‐sone‐mediated inhibition of plasminogen activator (PA) production by human‐embryonic lung (HuEL) cells has been examined. The enzyme produced by HuEL cells in the presence of both dexamethasone and actinomycin D appears to be

Volume changes of mammalian cells subjec

Volume changes of mammalian cells subjected to hypotonic solutions in vitro: Evidence for the requirement of a sodium pump for the shrinking phase

✍ Howard M. Rosenberg; Brenda Buckhold Shank; Earle C. Gregg 📂 Article 📅 1972 🏛 John Wiley and Sons 🌐 English ⚖ 724 KB

## Abstract A Coulter‐orifice pulse‐height analyzer system was used to measure volume spectra of mammalian cells in suspension at different times after the addition of an equal volume of water. In appropriate hypotonic medium, cultured mammalian cells rapidly increase in volume and then shrink, mor

Evidence for the presence of Thy-1 on cu

Evidence for the presence of Thy-1 on cultured thymic epithelial cells of mice and rats

✍ A. Raedler; R. Arndt; Elisabeth Raedler; Dorothee Jablonski; H.-G. Thiele 📂 Article 📅 1978 🏛 John Wiley and Sons 🌐 English ⚖ 904 KB

## Abstract Thymic tissue of C57BL/6 mice and DA rats was cultured. After 6–8 weeks, cultures were analyzed for their capacity to absorb anti‐Thy‐1 serum, and for the expression of Thy‐1 on the surface of different cell types by means of the indirect peroxidase labeling method. Three main cell type

T cell requirements for the expression o

T cell requirements for the expression of the lipopolysaccharide adjuvant effect in vivo: evidence for a T cell-dependent and a T cell-independent mode of action

✍ Dolly B. Ness; S. Smith; J. A. Talcott; F. C. Grumet 📂 Article 📅 1976 🏛 John Wiley and Sons 🌐 English ⚖ 537 KB

## Abstract The __in vivo__ adjuvant effect of lipopolysaccharide (LPS) in mice was investigated with the soluble synthetic polypeptide antigen (T, G)‐A–L, the antibody response to which is determined by the Ir‐1A gene. With this specific antigen it can be demonstrated that the LPS adjuvant effect

Immunofluorescent and immunochemical evi

Immunofluorescent and immunochemical evidence for the expression of cytovillin in the microvilli of a wide range of cultured human cells

✍ Raimo Pakkanen 📂 Article 📅 1988 🏛 John Wiley and Sons 🌐 English ⚖ 738 KB

We have previously purified a M, 75,000 protein, cytovillin, from cultured human choriocarcinoma cells (JEG-3) and shown that this protein was specifically confined to the microvillus membrane of these cells. I have now studied the expression and the subcellular distribution of cytovillin in eightee