Evidence for the presence of Thy-1 on cultured thymic epithelial cells of mice and rats
✍ Scribed by A. Raedler; R. Arndt; Elisabeth Raedler; Dorothee Jablonski; H.-G. Thiele
- Publisher
- John Wiley and Sons
- Year
- 1978
- Tongue
- English
- Weight
- 904 KB
- Volume
- 8
- Category
- Article
- ISSN
- 0014-2980
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
Thymic tissue of C57BL/6 mice and DA rats was cultured. After 6–8 weeks, cultures were analyzed for their capacity to absorb anti‐Thy‐1 serum, and for the expression of Thy‐1 on the surface of different cell types by means of the indirect peroxidase labeling method. Three main cell types were identified: epithelium‐like cells, fibrocyte‐like cells and macrophages, but no lymphocytes were found. The presence of Thy‐1 on cultured nonlymphocytic thymus‐derived cells was demonstrated by their ability to absorb the cytotoxic activity of the appropriate anti‐Thy‐1 sera. Electron microscopical analyses of labeling experiments revealed that Thy‐1 was predominantly expressed on epithelium‐like cells with preference for their cell protrusions.
The possible role of Thy‐1 expression, both on thymocytes and thymus epithelium for cellular interaction, is discussed.
📜 SIMILAR VOLUMES
## Abstract The molecular basis of water‐permeability in salivary and other exocrine glands is not understood. We have examined two well‐studied salivary epithelial cell lines for evidence of a Hg‐inhibitable water‐permeability pathway. A5 and HSG cells are derived from rat and human submandibular
## Abstract The presence of receptors for IgG Fc and fragments of C3 on primary cultures and cryostat sections of normal and rheumatoid synovial tissues was assessed. Significant proportions of large rounded cells with asteroid projections found in such cultures had receptors for both IgG Fc and fr