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Evaluation of Taurine Metabolism in Cats by Dual Stable Isotope Analysis

✍ Scribed by L.B. Fay; S. Métairon; F. Montigon; O. Ballèvre

Publisher
Elsevier Science
Year
1998
Tongue
English
Weight
115 KB
Volume
260
Category
Article
ISSN
0003-2697

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✦ Synopsis

Taurine kinetics in cats was investigated using a bolus dose of [ 15 N]-and [1,2-13 C 2 ]taurine. The comparison of [ 15 N]-and [ 13 C 2 ]taurine kinetics permitted an evaluation of the extent of taurine transamination. A methodology which involves N-pentafluorobenzoyl din-butylamine derivatization of taurine and GC/MS measurements of the 15 N-and 13 C-enrichments in cat urine was developed. Accuracy of the measurements was determined using pure standard compounds and the results showed that [ 13 C 2 ]taurine does not interfere with [ 15 N]taurine. In cats, no differences were observed between both tracers. Therefore, we conclude that taurine reversible transamination does not occur at a significant level in cats.

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