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Analysis of S-methylmethionine and S-adenosylmethionine in plant tissue by a dansylation, dual-isotope method

✍ Scribed by Peter K. Macnicol

Publisher
Elsevier Science
Year
1986
Tongue
English
Weight
612 KB
Volume
158
Category
Article
ISSN
0003-2697

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✦ Synopsis

A method is presented for determining the levels of S-methylmethionine (MeMet) and S-adenosylmethionine (AdoMet) in the same plant tissue sample, utilizing readily available equip ment. The bottom limit of sensitivity, ca. 100 pmol, can be lowered if required. A trichloracetic acid homogenate of the tissue is supplemented with [carboxyl-'%]MeMet and [carboxyl-?]AdoMet.

After separation of MeMet and AdoMet from each other and from endogenous homoserine on a phosphocellulose column, the two fractions are heat treated at appropriate pH values to liberate ['4C]homoserine. Quantitation is via the 'H/l% ratio of ['Hldansyl-['4C]homoserine isolated by thin-layer chromatography. The method is validated with pea cotyledon, corn root, and CaUliflOWer leaf. 0 1986 Academic Press, IIX.

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