Evaluation of matrix scaffolds for tissue engineering of articular cartilage grafts

✍ Scribed by Grande, D. A. ;Halberstadt, C. ;Naughton, G. ;Schwartz, R. ;Manji, Ryhana

• Publisher: John Wiley and Sons
• Year: 1997
• Tongue: English
• Weight: 675 KB
• Volume: 34
• Category: Article
• ISSN: 0021-9304
• DOI: 10.1002/(sici)1097-4636(199702)34:2<211::aid-jbm10>3.0.co;2-l

✦ Synopsis

Injury to articular cartilage predisposes that joint to further chondrocytes responded to culture on the various materials. degeneration and eventually osteoarthritis. Recent studies Bioabsorbable polymers such as polyglycolic acid (PGA)have demonstrated the feasibility of using chondrocytes to-enhanced proteoglycan synthesis, whereas collagen matrices gether with different biomaterial carriers as grafts for the stimulated synthesis of collagen. The use of the closed culture repair of cartilage defects. The following study was under-system, in general, improved the rates of synthesis of collagen taken to determine the effect of a variety of these materials and proteoglycan on the different material scaffolds. Excepon chondrocyte growth and extracellular matrix synthesis.

tions were collagen synthesis on collagen matrices: use of We cultured chondrocytes on several commonly used materi-the closed culture system did not enhance the rate of syntheals and compared their rates of synthesis of proteoglycan sis. Rates of proteoglycan synthesis on PGA scaffold initially and collagen. Additionally, we evaluated them in a closed was higher in the closed culture system but did not sustain culture recirculating system on these materials and compared a difference over the entire course of the 3-week culture them with standard culture techniques. This was done to period. This study demonstrates the importance of carrier see whether such a bioreactor-type system can be used to material for the purpose of cartilage tissue reconstruction in enhance the quality of in vitro reconstructed tissues. Our vitro.