Effects of growth factors on heparin-carrying polystyrene-coated atelocollagen scaffold for articular cartilage tissue engineering
✍ Scribed by Masato Sato; Masayuki Ishihara; Miya Ishihara; Nagatoshi Kaneshiro; Genya Mitani; Toshihiro Nagai; Toshiharu Kutsuna; Takashi Asazuma; Makoto Kikuchi; Joji Mochida
- Publisher
- John Wiley and Sons
- Year
- 2007
- Tongue
- English
- Weight
- 309 KB
- Volume
- 83B
- Category
- Article
- ISSN
- 1552-4973
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✦ Synopsis
Abstract
The specific aim of our investigation is to study the potential use of a collagen/heparin‐carrying polystyrene (HCPS) composite extracellular matrix for articular cartilage tissue engineering. Here, we created a high‐performance extracellular matrix (HpECM) scaffold to build an optimal extracellular environment using an HCPS we originally developed, and an atelocollagen honeycomb‐shaped‐scaffold (ACHMS‐scaffold) with a membrane seal. This scaffold was coated with HCPS to enable aggregation of heparin‐binding growth factors such as FGF‐2 and TGF‐β1 within the scaffold. Three‐dimensional culture of rabbit articular chondrocytes within the HpECM‐scaffold and subsequent preparation of a tissue‐engineered cartilage were investigated. The results showed remarkably higher cell proliferative activity within the HpECM‐pretreated‐FGF‐2 scaffold and the sustenance of phenotype within the HpECM‐pretreated‐TGF‐β1 scaffold. It was thought that both FGF‐2 and TGF‐β1 were stably immobilized in the HpEMC‐scaffold since HCPS generated an extracellular environment similar to that of heparan sulfate proteoglycan within the scaffold. These results suggest that an ACHMS‐scaffold immobilized with HCPS can be a HpECM for cartilage regeneration to retain the heparin‐binding growth factors within the scaffolds. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2007