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Evaluation of fluorometric and zymographic methods as activity assays for stromelysins and gelatinases

โœ Scribed by Ana R. Quesada; M. Mar Barbacid; Emilia Mira; Piedad Fernandez-Resa; Gabriel Marquez; Miguel Aracil

Book ID
110371655
Publisher
Springer
Year
1996
Tongue
English
Weight
64 KB
Volume
15
Category
Article
ISSN
0262-0898

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Prostaglandin H synthetase (PGH synthetase) has been purified to homogeneity from sheep vesicular glands. The pure enzyme has a specific activity of about 40 @.I of arachidonic acid consumed per minute per milligram of protein, which corresponds to a turnover number of 2800 mitt-' per subunit. The p