A sensitive tluorometric assay for DNA determination using m-diaminobenzoic acid dihydrochloride for the reaction with deoxyribose liberated by perchloric acid is described. Fluorescence is proportional to the amount of DNA over the range 0.05-5 pg of DNA when the reaction is conducted in a volume o
A rapid and sensitive fluorometric assay method for the determination of nitrilase activity
โ Scribed by Anirban Banerjee; Rohit Sharma; Uttam Chand Banerjee
- Book ID
- 111717919
- Publisher
- Portland Press
- Year
- 2003
- Tongue
- English
- Weight
- 97 KB
- Volume
- 37
- Category
- Article
- ISSN
- 0885-4513
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
Phospholipase A 2 (PLA 2 ) is an enzyme that cleaves fatty acids from the sn-2 position of membrane phospholipids. Intracellular PLA 2 has been shown to be important for the generation of arachidonic acid, which leads to the synthesis of a wide range of eicosanoid paracrine hormones. Furthermore, se
Most of the methods currently available for the determination of RNase activity are dependent on the measurement of acid-soluble products released 'by hydrolysis of RNA. The major advantages of these methods are that they are sensitive, reproducible, and convenient for large numbers of samples (l-3)
A rapid and sensitive fluorometric assay for the measurement of carnitine palmitoyltransferase (CPT) activity is described. In this assay the coenzyme A (CoA) liberated from palmitoyl-CoA by CPT reacts with N-(9-acridinyl)-maleimide to form a fluorescent product. By comparing the fluorescence intens