Carnitine palmitoyltransferase II (CPT-II) mediates the import of long-chain fatty acids into the mitochondrial matrix for subsequent โค-oxidation. Defects of CPT-II manifest as a severe neonatal hepatocardiomuscular form or as a mild muscular phenotype in early infancy or adolescence. CPT-II deficie
โฆ LIBER โฆ
Endpoint Fluorometric Assays for Determining Activities of Carnitine Palmitoyltransferase and Citrate Synthase
โ Scribed by R.Patrick Hassett; Elizabeth L. Crockett
- Publisher
- Elsevier Science
- Year
- 2000
- Tongue
- English
- Weight
- 56 KB
- Volume
- 287
- Category
- Article
- ISSN
- 0003-2697
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We report a novel fluorometric end-point assay for the determination of 1-deoxy-D-xylulose 5-phosphate synthase (DXS) activity based on the reaction of 1-deoxy-D-xylulose 5-phosphate (DX5P) with 3,5-diaminobenzoic acid in an acidic medium to form a highly fluorescent quinaldine derivative. The assay