Evaluation of determination of 6-aminopenicillanic acid byp-dimethyl aminobenzaldedyde
โ Scribed by Jaiprakash G. Shewale; Kamalesh K. Kumar; Gangadhar R. Ambekar
- Publisher
- Springer-Verlag
- Year
- 1987
- Tongue
- English
- Weight
- 197 KB
- Volume
- 1
- Category
- Article
- ISSN
- 0951-208X
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A new, sensitive calorimetric method for the estimation of 6-aminopenicillanic acid is described. The procedure is based upon formation of a 2,4-pentanedione derivative of 6-aminopenicillanic acid followed by a second reaction with p-dimethylaminobenzaldehyde (Ehrlich's reagent), resulting in a red
A high-performance liquid chromatographic method has been developed for the determination of 6-aminopenicillanic acid in amino acid mixtures and human serum. The separation of 6-aminopenicillanic acid was carried out on a C18 column using sodium heptylsulfonate or tetrabutylammonium bromide as an io
A quantitative GLC determination of 6-aminopenicillanic acid and 7-amino-3-methyl-delta3-cephem-4-carboxylic acid is presented. The results obtained are in good agreement with those of known chemical procedures. The method is free from interference by related substances.
Yields of colored Schiff base products of the reversible condensation of 6 -aminopenicillanic and 7 -aminocephalosporanic acids with para-dimethylaminobenzaldehyde in a weakly acid aqueous solution strongly increase in the presence of micelles of sodium dodecyl sulfate. The binding constants of reac