Evaluation and characterization ofTrichoderma reeseicellulase and xylanase promoters
β Scribed by Zinnia Rahman; Yosuke Shida; Takanori Furukawa; Yota Suzuki; Hirofumi Okada; Wataru Ogasawara; Yasushi Morikawa
- Book ID
- 105951913
- Publisher
- Springer
- Year
- 2009
- Tongue
- English
- Weight
- 356 KB
- Volume
- 82
- Category
- Article
- ISSN
- 1432-0614
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The production of cellulase and xylanase was investigated with a newly isolated strain of Tricho- derma viride BT 2169. The medium composition was optimized on a shake-flask scale using the Graeco-Latin square technique. The temperature and time for optimal growth and production of the enzymes in sh
Xylanase (fl-l,4-D-xylan xylanohydrolase; EC 3.2.1.8) from aleurone layers of barley (Hordeum vulgare L. cv. Himalaya) was purified and characterized. Purification was by preparative isoelectric focusing and a Sephadex G-200 column. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the en