The production of cellulase and xylanase was investigated with a newly isolated strain of Tricho- derma viride BT 2169. The medium composition was optimized on a shake-flask scale using the Graeco-Latin square technique. The temperature and time for optimal growth and production of the enzymes in shake cultures were optimized using a central composite design. The temperature optima for maximal production β’ of filter paper cellulase (FPase), xylanase and fl-gluosidase were 32.8 Β°, 34.7 Β° and 31.1 Β° C, respectively, and the optimum times for production of these enzymes were found to be 144, 158 and 170 h, respectively. The optimized culture medium and conditions (33Β°C) gave 0.55 unit of FPase, 188.1 units of xylanase and 3.37 units of fl-glucosidase per milliliter of culture filtrate at 144 h of shake culture. Among different carbon sources tested, the maximum enzyme activities were produced with sulphite pulp and all three enzymes were produced irrespective of the carbon sources used. Batch fermentation in a laboratory fermentor using 2% sulphit e pulp allowed the production of 0.61 unit of FPase, 145.0 units of xylanase and 2.72 units of fl-glucosidase. In a fed-batch fermentation on 6% final Avicel concentration FPase and fl-glucosidase were 3.0 and 2.4 times higher respectively than those in batch fermentation on 2% Avicel. The pH and temperature optima as well as pH and temperature stabilities of T. vir- ide enzymes were found to be comparable to T. reesei and some other fungal enzymes.