Studies on a wild strain of Schizophyllum commune: Cellulase and xylanase production and formation of the extracellular polysaccharide Schizophyllan
✍ Scribed by W. Steiner; R. M. Lafferty; I. Gomes; H. Esterbauer
- Publisher
- John Wiley and Sons
- Year
- 1987
- Tongue
- English
- Weight
- 838 KB
- Volume
- 30
- Category
- Article
- ISSN
- 0006-3592
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✦ Synopsis
A wild strain of Schizophyllum commune (Fr:Fr:) isolated in Bangladesh produced cellulase and xylanase in high yields as well as the exobiopolymer schizophyllan. It was found experimentally that concentrations of 4% Avicel, 3.5% peptone, and 0.5% Ca(N0J2-4H20 were optimal for growth and product formation. Bacto-peptone was found to be the most suitable substrate of a number of casein, mycological, and meat peptone preparations for enzyme production. Young plate-culture inocula (4 days) were found to be better than comparatively aged fungal cultures (14 days). With the optimized medium, 5 units filter paper (FP) cellulase, 1244 units xylanase, 108 units pglucosidase, and 65 units of carboxymethyl (CM) cellulase per m L culture filtrate were obtained in shake flasks.
In a laboratory fermentor the respective enzyme activities were 4.5 units FP-cellulase, 1200 units xylanase, 100 units P-glucosidase, and 60 units CM-cellulase per mL culture filtrate. A biopolymer, reported to be active against cancerous cells, was an additional product in addition t o the enzymes.