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Etude de structures peptidiques à l'aide du phénylisothiocyanate II. Sur l'emploi de la microchromatographie d'adsorption sur couches minces des phénylthiohydantoïnes

✍ Scribed by Emile Cherbuliez; Br. Baehler; J. Rabinowitz

Publisher
John Wiley and Sons
Year
1960
Tongue
German
Weight
199 KB
Volume
43
Category
Article
ISSN
0018-019X

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✦ Synopsis

Abstract

The microchromatography of phenylthiohydantoins on thin layers of silica (solvent: heptane, pyridine, ethyl acetate 5:3:2 vol.; revelation by starch‐iodineazide) is described. This method is 10 to 20 times more sensitive than that of classic paper chromatography. Good results are obtained in the EDMAN degradation of 0,01 μmole of di‐ or tri‐peptides.

📜 SIMILAR VOLUMES

Etude de structures peptidiques à l'aide
Etude de structures peptidiques à l'aide de phénylisothiocyanate V. Sur la formation des phénylthiohydantoïnes-[35S] et sur leur chromatographie
✍ Emile Cherbuliez; B. R. Baehler; J. Marszalek; A. R. Sussmann; J. Rabinowitz 📂 Article 📅 1963 🏛 John Wiley and Sons 🌐 German ⚖ 320 KB

## Abstract Using a solution of [^35^S]‐phenylisothiocyanate in pentane with a specific activity of 30 to 8O mC/mmole, the authors have determined the sequence of di‐, tri‐peptides and a pentapeptide on the following quantities: 1 to 2 nmole of peptide when the [^35^S]‐phenylthiohydantoines obtaine

Etude de structures peptidiques à l'aide
Etude de structures peptidiques à l'aide de phénylisothiocyanate III. Sur la formation des phénylthiohydantoïnes-[35S] et sur leur chromatographie sur papier
✍ Emile Cherbuliez; A. R. Sussmann; J. Rabinowitz 📂 Article 📅 1961 🏛 John Wiley and Sons 🌐 German ⚖ 290 KB

By the analysis of di‐, tri‐peptides and a pentapeptide of known constitution the authors show that using an N,N‐dimethylformamide solution of [^35^S]‐phenylisothiocyanate with a spectific activity of 10 to 40 mc/mmole the sequence of amino acids can be determined starting with 0,005 micromole of pe

Etude de structures peptidiques à l'aide
Etude de structures peptidiques à l'aide de phénylisothiocyanate IV. Note sur la préparation et la purification des phénylthiohydrantoïnes dérivées de divers acides aminés
✍ Emile Cherbuliez; J. Marszalek; J. Rabinowitz 📂 Article 📅 1963 🏛 John Wiley and Sons 🌐 German ⚖ 257 KB

## Abstract The authors describe modified procedures for the synthesis of phenylthiohydantoins derived from α‐amino acids, applied to 16 amino acids. With the exception of the phenylthiohydantoins containing a free NH~2~, CONH~2~, COOH or hetero‐cyclic group belonging to the starting amino acid,