𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Etude de structures peptidiques à l'aide de phénylisothiocyanate III. Sur la formation des phénylthiohydantoïnes-[35S] et sur leur chromatographie sur papier

✍ Scribed by Emile Cherbuliez; A. R. Sussmann; J. Rabinowitz

Publisher
John Wiley and Sons
Year
1961
Tongue
German
Weight
290 KB
Volume
44
Category
Article
ISSN
0018-019X

No coin nor oath required. For personal study only.

✦ Synopsis

By the analysis of di‐, tri‐peptides and a pentapeptide of known constitution the authors show that using an N,N‐dimethylformamide solution of [^35^S]‐phenylisothiocyanate with a spectific activity of 10 to 40 mc/mmole the sequence of amino acids can be determined starting with 0,005 micromole of peptide.

📜 SIMILAR VOLUMES

Etude de structures peptidiques à l'aide
Etude de structures peptidiques à l'aide de phénylisothiocyanate V. Sur la formation des phénylthiohydantoïnes-[35S] et sur leur chromatographie
✍ Emile Cherbuliez; B. R. Baehler; J. Marszalek; A. R. Sussmann; J. Rabinowitz 📂 Article 📅 1963 🏛 John Wiley and Sons 🌐 German ⚖ 320 KB

## Abstract Using a solution of [^35^S]‐phenylisothiocyanate in pentane with a specific activity of 30 to 8O mC/mmole, the authors have determined the sequence of di‐, tri‐peptides and a pentapeptide on the following quantities: 1 to 2 nmole of peptide when the [^35^S]‐phenylthiohydantoines obtaine

Etude de structures peptidiques à l'aide
Etude de structures peptidiques à l'aide du phénylisothiocyanate II. Sur l'emploi de la microchromatographie d'adsorption sur couches minces des phénylthiohydantoïnes
✍ Emile Cherbuliez; Br. Baehler; J. Rabinowitz 📂 Article 📅 1960 🏛 John Wiley and Sons 🌐 German ⚖ 199 KB

## Abstract The microchromatography of phenylthiohydantoins on thin layers of silica (solvent: heptane, pyridine, ethyl acetate 5:3:2 vol.; revelation by starch‐iodineazide) is described. This method is 10 to 20 times more sensitive than that of classic paper chromatography. Good results are obtain

Etude de structures peptidiques à l'aide
Etude de structures peptidiques à l'aide de phénylisothiocyanate IV. Note sur la préparation et la purification des phénylthiohydrantoïnes dérivées de divers acides aminés
✍ Emile Cherbuliez; J. Marszalek; J. Rabinowitz 📂 Article 📅 1963 🏛 John Wiley and Sons 🌐 German ⚖ 257 KB

## Abstract The authors describe modified procedures for the synthesis of phenylthiohydantoins derived from α‐amino acids, applied to 16 amino acids. With the exception of the phenylthiohydantoins containing a free NH~2~, CONH~2~, COOH or hetero‐cyclic group belonging to the starting amino acid,