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Etude de structures peptidiques à l'aide de phénylisothiocyanate V. Sur la formation des phénylthiohydantoïnes-[35S] et sur leur chromatographie

✍ Scribed by Emile Cherbuliez; B. R. Baehler; J. Marszalek; A. R. Sussmann; J. Rabinowitz

Publisher
John Wiley and Sons
Year
1963
Tongue
German
Weight
320 KB
Volume
46
Category
Article
ISSN
0018-019X

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✦ Synopsis

Abstract

Using a solution of [^35^S]‐phenylisothiocyanate in pentane with a specific activity of 30 to 8O mC/mmole, the authors have determined the sequence of di‐, tri‐peptides and a pentapeptide on the following quantities: 1 to 2 nmole of peptide when the [^35^S]‐phenylthiohydantoines obtained in each step of EDMAN'S degradation are identified by classical paper chromatography; 0,l to 0,2 nmole of peptide when these [^35^S]‐phenylthiohydantoines are identified by silica thin layer chromatography.

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Etude de structures peptidiques à l'aide
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✍ Emile Cherbuliez; A. R. Sussmann; J. Rabinowitz 📂 Article 📅 1961 🏛 John Wiley and Sons 🌐 German ⚖ 290 KB

By the analysis of di‐, tri‐peptides and a pentapeptide of known constitution the authors show that using an N,N‐dimethylformamide solution of [^35^S]‐phenylisothiocyanate with a spectific activity of 10 to 40 mc/mmole the sequence of amino acids can be determined starting with 0,005 micromole of pe

Etude de structures peptidiques à l'aide
Etude de structures peptidiques à l'aide du phénylisothiocyanate II. Sur l'emploi de la microchromatographie d'adsorption sur couches minces des phénylthiohydantoïnes
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## Abstract The microchromatography of phenylthiohydantoins on thin layers of silica (solvent: heptane, pyridine, ethyl acetate 5:3:2 vol.; revelation by starch‐iodineazide) is described. This method is 10 to 20 times more sensitive than that of classic paper chromatography. Good results are obtain

Etude de structures peptidiques à l'aide
Etude de structures peptidiques à l'aide du phénylisothiocyanate-[35S]
✍ Emile Cherbuliez; Br. Baehler; M. C. Lebeau; A. R. Sussmann; J. Rabinowitz 📂 Article 📅 1960 🏛 John Wiley and Sons 🌐 German ⚖ 325 KB

## Abstract The use of [^35^S]‐phenylisothiocyanate (EDMAN's reagent, with radioactif tracer) for the determination of the sequence of amino acids in peptides is described. With an activity of 15 mc/mM in this reagent, quantities of 0.005 to 0.01 micromole of peptide (di‐ and tri‐peptides) can be u

Etude de structures peptidiques à l'aide
Etude de structures peptidiques à l'aide de phénylisothiocyanate IV. Note sur la préparation et la purification des phénylthiohydrantoïnes dérivées de divers acides aminés
✍ Emile Cherbuliez; J. Marszalek; J. Rabinowitz 📂 Article 📅 1963 🏛 John Wiley and Sons 🌐 German ⚖ 257 KB

## Abstract The authors describe modified procedures for the synthesis of phenylthiohydantoins derived from α‐amino acids, applied to 16 amino acids. With the exception of the phenylthiohydantoins containing a free NH~2~, CONH~2~, COOH or hetero‐cyclic group belonging to the starting amino acid,