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Estimation of the thickness of films used in electron microscopy

✍ Scribed by Dr. C. Weibull


Publisher
John Wiley and Sons
Year
2007
Tongue
English
Weight
210 KB
Volume
12
Category
Article
ISSN
0233-111X

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✦ Synopsis


Carbon films or thin sections of plastic materials were introduced into the electron beam of an electron microscope and the fraction of electrons scattered enough not to reach the fluorescent screen was estimated by means of the exposure meter of the microscope. The values obtained showed that a simple relationship existed between the fraction of electrons scattered and the thickness of the sections (films) AS estimated with a microtome or otherwise. I n this way, film thicknesses between about 5 and 130 n m can rapidly be estimated.

However, when sectioned material is studied, areas free of embedded material must be present in the sections.


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