Gel filtration, the chromatographic separation of proteins on columns of gel particles of various nature, has empirically been found to constitute a useful tool for molecular size determination (l-6), as indicated by a variety of applications. Few attempts have been made to utilize the interaction o
Estimation of molecular weights of small proteins on polyacrylamide gel electrophoresis
โ Scribed by Amnon Gonenne; Jacob Lebowitz
- Book ID
- 107711733
- Publisher
- Elsevier Science
- Year
- 1975
- Tongue
- English
- Weight
- 611 KB
- Volume
- 64
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
A nonlinear relationship between polyacrylamide gel electrophoresis (PAGE) retardation coefficients (KR) and molecular weights has been observed during analysis of several multimeric proteins. Although the deviation from linearity over a wide range of molecular weights is slight, it can lead to sign
A polyacrylamide gel elec&ophoresis system is described which employs the nonionic detergent T&on X-100 as a protein solvent. Aside from the obvious advantages of this detergent over ionic detergents and urea in the preservation of protein structure and function, it is demonstrated that the ancillar
The utilization of 2-methoxy-2,4-diphenyl-3(2H)-furanone (MDPF) as a fluorescent label for proteins in SDS polyacrylamide gel electrophresis is described. The procedure is very sensitive and detects disks containing as little as 1 ng of protein. The fluorescence signal is linear with concentration o