Estimation of Molecular Weights of Proteins by Gel Filtration
โ Scribed by ANDREWS, P.
- Book ID
- 109624157
- Publisher
- Nature Publishing Group
- Year
- 1962
- Tongue
- English
- Weight
- 445 KB
- Volume
- 196
- Category
- Article
- ISSN
- 0028-0836
- DOI
- 10.1038/196036a0
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๐ SIMILAR VOLUMES
Gel filtration, the chromatographic separation of proteins on columns of gel particles of various nature, has empirically been found to constitute a useful tool for molecular size determination (l-6), as indicated by a variety of applications. Few attempts have been made to utilize the interaction o
A method for analysis of elution data of proteins, obtained from Sephadex gel filtration experiments, is described. The relevant elution data from seven different proteins, with known molecular weights and Stoke's radii, were fitted into various equations relating elution parameters and molecular si