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The estimation of molecular weights of basic proteins (histones) by gel filtration in 6 M guanidine hydrochloride solution

✍ Scribed by J.M. Woynarowski; J. Konopa

Book ID
108335515
Publisher
Elsevier Science
Year
1973
Tongue
English
Weight
213 KB
Volume
77
Category
Article
ISSN
1873-3778

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A technique has been perfected for measuring the sedimentation coefficient of microgram quantities of a reduced protein in 6 M guanidine hydrochloride. The protein is sedimented through a gradient of 5-8 M guanidine-HCl in the presence of dithiothreitol in a SW 50.1 swinging-bucket rotor. Run condit