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Estimating the variation in S phase duration from flow cytometric histograms

✍ Scribed by Sara Larsson; Tobias Ryden; Ulla Holst; Stina Oredsson; Maria Johansson


Book ID
116733012
Publisher
Elsevier Science
Year
2008
Tongue
English
Weight
425 KB
Volume
213
Category
Article
ISSN
0025-5564

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S-phase fraction assessed by a variant o
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## Measurement of S-phase fraction by DNA flow cytometry is widely used in the analysis of human tumors. The calculation of fraction of S-phase cells is performed either by means of curve-fitting techniques or by more simple planimetric methods. Estimates by the latter often suffer from subjectivi

Different calculation methods for flow c
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S-phase fraction (SPF), estimated in the flow cytometric DNA histogram, is a prognostic factor in breast cancer. There are, however, some inherent difficulties in the estimation of SPF, such as the influence of debris, aggregates, and normal cells. Most of the available SPF calculation principles tr