## Measurement of S-phase fraction by DNA flow cytometry is widely used in the analysis of human tumors. The calculation of fraction of S-phase cells is performed either by means of curve-fitting techniques or by more simple planimetric methods. Estimates by the latter often suffer from subjectivi
Evaluation of the S phase distribution of flow cytometric DNA histograms by autoradiography and computer algorithms
β Scribed by L. E. Sheck; K. A. Muirhead; P. K. Horan
- Publisher
- John Wiley and Sons
- Year
- 1980
- Tongue
- English
- Weight
- 751 KB
- Volume
- 1
- Category
- Article
- ISSN
- 0196-4763
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## Abstract ## Background The value of flow cytometric analysis of DNA ploidy and Sβphase fraction (SPF) as an indicator of regional metastasis in oral cancer is currently being debated. Intratumoral heterogeneity makes this problem complex. ## Methods Intratumoral DNA ploidy heterogeneity and i
Conflicting prognostic results with regard to DNA flow cytometric cell cycle variables have been reported for breast cancer patients. An important reason for this may be related to differences in the interpretation of DNA histograms. Several computer programs based on different cell cycle fitting mo