Establishment of human fibroblast cell lines with lysosomal enzyme deficiency by transformation with origin-minus SV40 DNA
β Scribed by T. Furuya; T. Momoi; Y. Suzuki; H. Sato; N. Yamaguchi
- Publisher
- Springer
- Year
- 1985
- Tongue
- English
- Weight
- 272 KB
- Volume
- 8
- Category
- Article
- ISSN
- 0141-8955
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Co-culturing with origin-defective SV40 DNAinjected transformants, we succeeded in activating the limited growth potential of late-phase-II fibroblasts from patients with Werner's syndrome (WS cells). Residual life spans of three WS cell lines increased 2.3-5.6 fold. Co-culture with normal IMR-90 ce
## Abstract Human skin fibroblasts derived from patients with nephropathic cystinosis were transformed with SV40 virions, cloned and permitted to enter the degenerative stage of growth termed βcrisis,β characteristics of SV40 transformed human cells. Nephropathic cystinosis is an autosomal recessiv