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Establishment and characterization of a human urinary bladder carcinoma cell line (TSGH-8301)

✍ Scribed by Ming-Yang Yeh; Dah-Shyong Yü; Shan-Chun Chen; Ming-Shan Lin; Sun-Yran Chang; Cheng-Ping Ma; Shou-Hwa Han

Publisher
John Wiley and Sons
Year
1988
Tongue
English
Weight
682 KB
Volume
37
Category
Article
ISSN
0022-4790

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✦ Synopsis

A cell line derived from a well-differentiated human transitional cell carcinoma of the urinary bladder, designated TSGH-8301, was established in vitro. The cultured epithelioid cells exhibited monolayer growth and loss of contact inhibition. The tumorigenicity of TSGH-8301 had been shown by growth in soft agar and tumor induction in athymic nude mice. A reverse ratio of lactate dehydrogenase (LHD) isoenzyme in the cell line and nude mouse-grown tumors was seen predominantly with LDH-V. Chromosomal analysis revealed a heterodiploid stem line with a modal number of 50. Sera of urinary bladder cancer patients reacted with membrane antigens of the TSGH-8301 cells, suggesting the existence of tumorassociated antigens in the cells. In vitro chemosensitivity tests of these cells may provide data valuable in the selection of proper anticancer drugs for the TSGH-8301 donor patient.

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