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Erythroid and granulocytic colony growth in cultures supplemented with human serum lipoproteins

✍ Scribed by M. T. Aye; J. A. Seguin; J. P. McBurney

Publisher: John Wiley and Sons
Year: 1979
Tongue: English
Weight: 480 KB
Volume: 99
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1040990210

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✦ Synopsis

Abstract

The ability of human serum to support erythroid an dgranulocytic colony formation has been investigated. It was found that normal human serum could replace fetal calf serum in the cultures and was able to support the growth of these hemopoietic colonies. Serum fractions enriched for low density lipoproteins, either by precipitation with Heparin‐Mn^++^ or by ultra‐centrifugation, was found to contain this growth supporting activity of human serum.

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