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The synthesis of the Gγ and Aγ chains of human fetal hemoglobin in erythroid colonies cultured from peripheral blood BFUe's of normal adults and newborn and of subjects with an Aγ or a Gγ chain abnormal fetal hemoglobin

✍ Scribed by T. H. J. Huisman; A. L. Reese; M. E. Gravely; H. Harris; J. B. Wilson


Publisher
John Wiley and Sons
Year
1980
Tongue
English
Weight
879 KB
Volume
9
Category
Article
ISSN
0361-8609

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Hemoglobin biosynthesis in individual bu
✍ Takashi Terasawa; MD PhD Makio Ogawa 📂 Article 📅 1980 🏛 John Wiley and Sons 🌐 English ⚖ 479 KB 👁 1 views

## Abstract We cultured human adult peripheral and umbilical cord blood mononuclear cells in methylcellulose and measured the synthetic rates of globin chains in individual erythropoietic bursts. Globin chains were labeled with ^14^C‐amino acids in culture, separated by isoelectric focusing in slab