Ergosterol determination in Saccharomyces cerevisiae. Comparison of different methods
โ Scribed by M. Lamacka; J. Sajbidor
- Book ID
- 110371694
- Publisher
- Springer-Verlag
- Year
- 1997
- Tongue
- English
- Weight
- 162 KB
- Volume
- 11
- Category
- Article
- ISSN
- 0951-208X
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Data obtained from acid hydrolysis and extraction of yeast have demonstrated that routine saponification does not recover total sterol from the cells. This suggests the existence of a form of ergosterol resistant to saponification. Time course analyses of sterol synthesis by resting cell suspensions
Past work in this laboratory has shown that the stationary populations of 8. cerevisiae produced in a yeast extract medium during serial transfer under continuous anaerobic conditions mere quite small, while excellent growth occurred in the presence of oxygen (Brockmann and Stier, '47). It was also