Interest in accurate measurement of biotin concentrations in plasma and urine has been stimulated by recent advances in the understanding of biotin-responsive inborn errors of metabolism and by several reports describing acquired biotin deficiency during parenteral ahmentation. This paper presents a
β¦ LIBER β¦
Enzymic solid-phase assay for biotin and a biotin-benzodiazepine conjugate
β Scribed by Takeuchi, Toshifumi; Rechnitz, Garry A.
- Book ID
- 127264499
- Publisher
- American Chemical Society
- Year
- 1990
- Tongue
- English
- Weight
- 453 KB
- Volume
- 1
- Category
- Article
- ISSN
- 1043-1802
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The quenching of the tryptophan fluorescence of avidin by biotin forms the basis for a rapid, sensitive assay for biotin and avidin in solutions free of fluorescent contaminants. Avidin solutions are assayed by titration with a standard biotin solution until no further decrease in fluorescence at 35