A sequential, solid-phase assay for biotin in physiologic fluids that correlates with expected biotin status
✍ Scribed by Donald M. Mock; Dwight B. DuBois
- Publisher
- Elsevier Science
- Year
- 1986
- Tongue
- English
- Weight
- 548 KB
- Volume
- 153
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
Interest in accurate measurement of biotin concentrations in plasma and urine has been stimulated by recent advances in the understanding of biotin-responsive inborn errors of metabolism and by several reports describing acquired biotin deficiency during parenteral ahmentation. This paper presents a biotin assay utihzing radiolabeled avidin in a sequential, solid-phase method; the assay has increased sensitivity compared to previous methods (a10 fmol/tube), correlates with expected trends in biotin concentrations in blood and urine in a rat model of biotin deficiency, and can utilize commercially available radiolabeled avidin. Q 1986 Academic &ss, h.