𝔖 Bobbio Scriptorium
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Enzymes || Tight Binding Inhibitors

✍ Scribed by Copeland, Robert A.

Book ID
121081490
Publisher
John Wiley & Sons, Inc.
Year
2000
Weight
158 KB
Category
Article
ISBN-13
9780471220633

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High-Throughput Screening of Enzyme Inhi
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## Determination of tight-binding inhibition constants by nonlinear least-squares regression requires sufficiently good initial estimates of the best-fit values. Normally an initial estimate of the inhibition constant must be provided by the investigator. This paper describes an automatic procedur

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Active site titration by a reversible tight-binding inhibitor normally depends on prior knowledge of the inhibition constant. Conversely, the determination of tight-binding inhibition constants normally requires prior knowledge of the active enzyme concentration. Often, neither of these quantities i