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Mixtures of tight-binding enzyme inhibitors. Kinetic analysis by a recursive rate equation

✍ Scribed by Petr Kuzmič; Ka-Yun Ng; Timothy D. Heath

Book ID
107719049
Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
742 KB
Volume
200
Category
Article
ISSN
0003-2697

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Accurate Determination of Rate Constants of Very Slow, Tight-Binding Competitive Inhibitors by Numerical Solution of Differential Equations, Independently of Precise Knowledge of the Enzyme Concentration
✍ Igor W. Plesner; Anne Bülow; Mikael Bols 📂 Article 📅 2001 🏛 Elsevier Science 🌐 English ⚖ 108 KB

This paper is concerned with the determination of rate constants characterizing the binding and release of a slow binding inhibitor to and from an enzyme, here almond ␤-glucosidase. We demonstrate the inability of the conventional method to yield reliable rate constants when one or more of these is