Provides Key Information On A Wide Range Of Enzymes Commonly Used As Tools In Molecular Biology, Helping To Minimize The Time A Scientist Spends Researching The Literature To Get Reactions To Work Efficiently And Allowing The Nonenzymologist To Design An Experiment. Each Chapter Gives Background Information On The Enzyme Selected And Those Parameters Important In Its Use, Describes Both The Source And Application Of The Enzyme, And Provides Details On The Size And Structure Of The Protein. Specific Parameters Essential For Achieving An Optimized Reaction Are Discussed, Along With Exemplary Practical Procedures An Protocols. Nucleases: An Overview / A. Fred Weir -- Deoxyribonuclease I (ec 3.1.21.1) And Ii (ec 3.1.22.1) / A. Fred Weir -- Dna Polymerases (ec 2.7.7.7) / Martin J. Maunders -- Taq Polymerase (ec 2.7.7.7): With Particular Emphasis On Its Use In Pcr Protocols / Axel Landgraf And Heiner Wolfes -- Eukaryotic Nuclear Rna Polymerases (ec 2.7.7.6) / Deborah A. Cook And Robert J. Slater -- Reverse Transcriptase (ec 2.7.7.49): The Use Of Cloned Moloney Murine Leukemia Virus Reverse Transcriptase To Synthesize Dna From Rna / Gary F. Gerard And James M. D'alessio -- Terminal Deoxyribonucleotidyl Transferase (ec 2.7.7.31) / Frank Grosse And Andreas Manns -- Restriction Enzymes / Alfred Pingoud, Jurgen Alves And Robert Geiger -- Dna Methyltransferases (ec 2.1.1.72 And Ec 2.1.1.73) / David P. Hornby -- Dna And Rna Ligases (ec 6.5.1.1, Ec 6.5.1.2 And Ec 6.5.1.3) / Martin J. Maunders -- Bal 31 Nucleases (ec 3.1.11) / Horace B. Gray, Jr. And Tao Lu -- Mung-bean Nuclease 1 (ec 3.1.11) / Andrew J. Sharp And Robert J. Slater -- Rnase A (ec 3.1.27.5) / Michael M. Burrell -- Pronase (ec 3.4.24.4) / Patricia J. Sweeney And John M. Walker -- Proteolytic Enzymes For Peptide Production / Patricia J. Sweeney And John M. Walker -- Proteinase K (ec 3.4.21.14) / Patricia J. Sweeney And John M. Walker -- Carboxypeptidase Y (ec 3.4.16.1) / Julia S. Winder And John M. Walker -- Aminopeptidases: Aminopeptidase M (ec 3.4.11.2), Pyroglutamate Aminopeptidase (ec 3.4.19.3) And Prolidase (ec 3.4.13.9) / Patricia J. Sweeney And John M. Walker -- Alkaline Phosphatase (ec 3.1.3.1) / Martin J. Maunders -- Polynucleotide Kinase (ec 2.7.1.78) / Martin J. Maunders. Edited By Michael M. Burrell. Includes Bibliographical References And Index.